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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
19/06/2019 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALMEIDA, D.S.; PAZ, L.N.; DE OLIVEIRA, D.S.; RISTOW, P.; HAMOND, C.; COSTA, F.; PORTELA, R.W.; ESTRELA-LIMA, A.; PINNA, M.H. |
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Afiliación : |
Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Instituto Gonçalo Moniz, Ministério da Saúde, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil Silva, D.N. // Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Laboratory of Bacteriology and Health, Institute of Biology, Federal University of Bahia, Salvador, Bahia, Brazil.; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Saude Coletiva, Federal University of Bahia, Salvador, Brazil.; Instituto de Ciências da Saúde, Federal University of Bahia, Salvador, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil. |
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Título : |
Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
PloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391 |
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DOI : |
10.1371/journal.pone.0217391 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation
for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
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Contenido : |
ABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis. MenosABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected shee... Presentar Todo |
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Palabras claves : |
DISEASES OF SHEEP; LEPTOSPIRES; PLATAFORMA EN SALUD ANIMAL. |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES; LEPTOSPIROSIS. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16705/1/pone.0217391.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532964/pdf/pone.0217391.pdf
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Marc : |
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001 1059867
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008 2019 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0217391$2DOI
100 1 $aALMEIDA, D.S.
245 $aInvestigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
520 $aABSTRACT. The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis.
650 $aENFERMEDADES DE LOS ANIMALES
650 $aLEPTOSPIROSIS
653 $aDISEASES OF SHEEP
653 $aLEPTOSPIRES
653 $aPLATAFORMA EN SALUD ANIMAL
700 1 $aPAZ, L.N.
700 1 $aDE OLIVEIRA, D.S.
700 1 $aRISTOW, P.
700 1 $aHAMOND, C.
700 1 $aCOSTA, F.
700 1 $aPORTELA, R.W.
700 1 $aESTRELA-LIMA, A.
700 1 $aPINNA, M.H.
773 $tPloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391
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INIA La Estanzuela (LE) |
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| Registros recuperados : 15 | |
| 3. |  | COSTA, F.O.; VALENTE, T.S.; DEL CAMPO, M.; COSTA, M.J.R.P. Relationship between maternal defensiveness behavior and performance of Nellore calves. [Abstract.] Congreso de la Asociación Latinoamericana de Producción Animal, 24.; Congreso de la Sociedad Chilena de Producción Animal, 40., Puerto Varas, Chile, 9 al 13 noviembre 2015. ln: Reunión ALPA (24., Puerto Varas, Chile). Resúmenes. Puerto Varas (Chile): ALPA, 2015. p. 268| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 4. | | COSTA, F.O.; DEL CAMPO, M.; SOARES DE LIMA, J.M.; BRITO, G.; PARANHOS DA COSTA, M.J.R The effect of different pre slaughter procedures on carcas and meat quality in Uruguay. In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY (ICoMST), 60., 2014, Punta del Este, Uruguay. Proceedings... Montevideo: INIA, 2014.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Tacuarembó. |
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| 6. | | COSTA, F.D.O.; VALENTE, T.S.; DE TOLEDO, L.M.; AMBRÓSIO, L.A.; DEL CAMPO, M.; PARANHOS DA COSTA, M.J.R. A conceptual model of the human-animal relationships dynamics during newborn handling on cow-calf operation farms. Livestock Science, 2021, Volume 246, Article number 104462. Doi: https://doi.org/10.1016/j.livsci.2021.104462 Article history: Received 24 August 2020, Revised 23 February 2021, Accepted 24 February 2021, Available online 28 February 2021. Acknowledgements: We appreciated the support of Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
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| 7. | | COSTA, F.; BORGES, V.S.; CONDE, M.V.; SILVA, A.; VALENTE, T.; PARANHOS DA COSTA, M.J.R.; DEL CAMPO, M. Characterization of maternal protective behavior of Hereford cows and its effect on calf performance. [Resumen]. In: CONGRESO ASOCIACIÓN URUGUAYA DE PRODUCCIÓN ANIMAL (6º, Marzo, 2018, Tacuarembó, Uruguay). Resúmenes. Tacuarembó: AUPA, 2018.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Tacuarembó. |
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| 8. | | ALMEIDA, D.S.; PAZ, L.N.; DE OLIVEIRA, D.S.; RISTOW, P.; HAMOND, C.; COSTA, F.; PORTELA, R.W.; ESTRELA-LIMA, A.; PINNA, M.H. Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. PloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391 Article history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation
for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 9. | | COSTA, F. DE O.; BRITO, G.; SOARES DE LIMA, J.M.; SANT ´ANNA, A.C.; COSTA, M.J.R.P. DA; DEL CAMPO, M. Lairage time effect on meat quality in Hereford steers in rangeland conditions. Revista Brasileira de Zootecnia. 2019, 48(0) - OPEN ACCESS. Article History: Received: January 30, 2018 // Accepted: October 18, 2018 // Published online: May 13, 2019.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
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| 10. | | COSTA, F. O.; DEL CAMPO, M.; BRITO, G.; SOARES DE LIMA, J.M.; SANT´ANNA, A.; PARANHOS DA COSTA, M.J.R Lairage time and temperament effects on glycogen content in Hereford steers. In: International Congress of Meat Science and Technology, Meat for Global Sustainability, 62., 14-19th August 2016, Bangkok, Thailand.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Tacuarembó. |
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| 11. | | COSTA, F. O.; DEL CAMPO, M.; BRITO, G.; SOARES DE LIMA, J.M.; SANT´ANNA, A.; PARANHOS DA COSTA, M.J.R Lairage time and temperament effects on glycogen content in Hereford steers. [Poster]. In: International Congress of Meat Science and Technology, Meat for Global Sustainability, 62., 14-19th August 2016, Bangkok, Thailand.| Biblioteca(s): INIA Tacuarembó. |
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| 12. | | DEL CAMPO, M.; BRITO, G.; DE OLIVEIRA COSTA, F.; VERGARA, E.; ANCHAÑO, E.; FRUGONI, J.; BOTTERO, S.; LEVRATTO, J.; RODRIGUEZ, H.; HERNANDEZ, S.; ESCAYOLA, A.; OLIVERA, P. II. Efecto del manejo previo a la faena sobre el bienestar animal y la calidad de producto. Año 2. In: BERRETTA, E.; MONTOSSI, F.; BRITO, G. (Ed.). Alternativas tecnológicas para los sistemas ganaderos del basalto. Montevideo, UY: INIA, 2014. p. 539-554 (Serie Técnica; 217)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó; INIA Treinta y Tres. |
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| 13. | | CASANOVAS-MASSANA, A.; HAMOND, C.; SANTOS, L.A.; DE OLIVEIRA, D.; HACKER, K.P.; BALASSIANO, I.; COSTA, F.; MEDEIROS, M.A.; REIS, M.G.; KO, A.I.; WUNDER, E.A. Leptospira yasudae sp. nov. and Leptospira stimsonii sp. nov., two new species of the pathogenic group isolated from environmental sources. International Journal of Systematic and Evolutionary Microbiology, 1 March 2020, Volume 70, Issue 3, Pages 1450-1456. OPEN ACCESS. Doi: https://doi.org/10.1099/ijsem.0.003480 Article history: First Published: 11 June 2019//Correspondence: Arnau Casanovas-Massana, arnau.casanovas@yale.edu. Funding information:This research was supported by the National Institutes of Health research grants R01 AI052473, U01...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 14. | | DEBLITZ, C.; BORBELY, C.; CHARRY, A.A.; COSTA, F.P.; VON DAVIER, Z.; FERNANDEZ, E.; GRABNER, R.; HASHMI, A.H.; IZQUIERDO, L.; JAKOBE, P.; KLAPKA, J.; MAHMOOD, K.; OSTROWSKI, B.; PARTON. K.; PEREIRA, M.; RAS, C.H.; REYES, E.; RICHARDSON, J.W.; SARZEAUD, P.; SWITLYK, M.; SZABO, K.; THORNE, F.; WILCZYNSKI, A. Status quo and prospects of beef production world-wide. (Review) Landbauforschung Völkenrode, December 2004, Volume 54, Issue 4, Pages 237-249. 2-s2.0-9944252565 Article history:| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 1 |
| Biblioteca(s): INIA Las Brujas. |
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| 15. | | ROEL, A.; FLORES, C.A.; HIROO SAITO, C.; HELLNVIG ZARNOTT, D.; MUSITELLI ANDREASEN, D.; AMORIM DA COSTA, F.; EVIA PICCIOLI, G.; DE FIGUEIREDO NACHTIGAL, G.; RODRÍGUEZ DE ALMEIDA, I.; MENEGHETI, J.O.; IEIRA SOBRINHO, J.P.; HENRIQUE CARDOSO, J.; FILIPPINI ALBA, J.M.; WINCKLER SOSINSKI, L.T.; VILARÓ VARELA, M.; BARBIERI, R.L.; GOUVEA, T.; STEIKE, V. Sustentabilidade socioambiental da bacia da Lagoa Mirim.[Actas]. WORKSHOP INTERNACIONAL, 1., 2009, PELOTAS, RS, BRASIL. Pelotas, RS (Brasil): Embrapa Clima Temperado, 2010. 292 p.| Biblioteca(s): INIA Treinta y Tres. |
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| Registros recuperados : 15 | |
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